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rnascope 2.5 hd duplex detection kit (chromogenic)  (Advanced Cell Diagnostics Inc)


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    Advanced Cell Diagnostics Inc rnascope 2.5 hd duplex detection kit (chromogenic)
    Rnascope 2.5 Hd Duplex Detection Kit (Chromogenic), supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope+2%2E5+hd+duplex+detection+kit+(chromogenic)/rnascope+multiplex+fluorescent+reagent+kit+v2/bio_rxiv__2025__07__15__664994-144-11-22
    Average 90 stars, based on 1 article reviews
    rnascope 2.5 hd duplex detection kit (chromogenic) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Optimized GMP-grade production of non-viral Sleeping Beauty-generated CARCIK cells for enhanced fitness and clinical scalability.
    Article Snippet: lex RNAscope® probes for CAR-CIK (Channel 1, green) and CD19 (Channel 2, red) were hybridized at 40 °C for 2 h using the HybEZ™ Hybridization System II. Signal amplification and detection utilized the RNAscope® Kit 2.5 HD Duplex Detection Kit (Cat. 322500), followed by red and green chromogenic revelation and Gill’s hematoxylin nuclear counterstaining. Slides were mounted using Vectamount® medium.

    RNAscope:

    Article Title: Optimized GMP-grade production of non-viral Sleeping Beauty-generated CARCIK cells for enhanced fitness and clinical scalability.
    Article Snippet: lex RNAscope® probes for CAR-CIK (Channel 1, green) and CD19 (Channel 2, red) were hybridized at 40 °C for 2 h using the HybEZ™ Hybridization System II. Signal amplification and detection utilized the RNAscope® Kit 2.5 HD Duplex Detection Kit (Cat. 322500), followed by red and green chromogenic revelation and Gill’s hematoxylin nuclear counterstaining. Slides were mounted using Vectamount® medium.

    Hybridization:

    Article Title: Optimized GMP-grade production of non-viral Sleeping Beauty-generated CARCIK cells for enhanced fitness and clinical scalability.
    Article Snippet: lex RNAscope® probes for CAR-CIK (Channel 1, green) and CD19 (Channel 2, red) were hybridized at 40 °C for 2 h using the HybEZ™ Hybridization System II. Signal amplification and detection utilized the RNAscope® Kit 2.5 HD Duplex Detection Kit (Cat. 322500), followed by red and green chromogenic revelation and Gill’s hematoxylin nuclear counterstaining. Slides were mounted using Vectamount® medium.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Optimized GMP-grade production of non-viral Sleeping Beauty-generated CARCIK cells for enhanced fitness and clinical scalability.
    Article Snippet: lex RNAscope® probes for CAR-CIK (Channel 1, green) and CD19 (Channel 2, red) were hybridized at 40 °C for 2 h using the HybEZ™ Hybridization System II. Signal amplification and detection utilized the RNAscope® Kit 2.5 HD Duplex Detection Kit (Cat. 322500), followed by red and green chromogenic revelation and Gill’s hematoxylin nuclear counterstaining. Slides were mounted using Vectamount® medium.

    Incubation:

    Article Title: Optimized GMP-grade production of non-viral Sleeping Beauty-generated CARCIK cells for enhanced fitness and clinical scalability.
    Article Snippet: lex RNAscope® probes for CAR-CIK (Channel 1, green) and CD19 (Channel 2, red) were hybridized at 40 °C for 2 h using the HybEZ™ Hybridization System II. Signal amplification and detection utilized the RNAscope® Kit 2.5 HD Duplex Detection Kit (Cat. 322500), followed by red and green chromogenic revelation and Gill’s hematoxylin nuclear counterstaining. Slides were mounted using Vectamount® medium.

    Control:

    Article Title: Optimized GMP-grade production of non-viral Sleeping Beauty-generated CARCIK cells for enhanced fitness and clinical scalability.
    Article Snippet: lex RNAscope® probes for CAR-CIK (Channel 1, green) and CD19 (Channel 2, red) were hybridized at 40 °C for 2 h using the HybEZ™ Hybridization System II. Signal amplification and detection utilized the RNAscope® Kit 2.5 HD Duplex Detection Kit (Cat. 322500), followed by red and green chromogenic revelation and Gill’s hematoxylin nuclear counterstaining. Slides were mounted using Vectamount® medium.

    Staining:

    Article Title: Optimized GMP-grade production of non-viral Sleeping Beauty-generated CARCIK cells for enhanced fitness and clinical scalability.
    Article Snippet: lex RNAscope® probes for CAR-CIK (Channel 1, green) and CD19 (Channel 2, red) were hybridized at 40 °C for 2 h using the HybEZ™ Hybridization System II. Signal amplification and detection utilized the RNAscope® Kit 2.5 HD Duplex Detection Kit (Cat. 322500), followed by red and green chromogenic revelation and Gill’s hematoxylin nuclear counterstaining. Slides were mounted using Vectamount® medium.

    Confocal Microscopy:

    Article Title: Optimized GMP-grade production of non-viral Sleeping Beauty-generated CARCIK cells for enhanced fitness and clinical scalability.
    Article Snippet: lex RNAscope® probes for CAR-CIK (Channel 1, green) and CD19 (Channel 2, red) were hybridized at 40 °C for 2 h using the HybEZ™ Hybridization System II. Signal amplification and detection utilized the RNAscope® Kit 2.5 HD Duplex Detection Kit (Cat. 322500), followed by red and green chromogenic revelation and Gill’s hematoxylin nuclear counterstaining. Slides were mounted using Vectamount® medium.

    Microscopy:

    Article Title: Optimized GMP-grade production of non-viral Sleeping Beauty-generated CARCIK cells for enhanced fitness and clinical scalability.
    Article Snippet: lex RNAscope® probes for CAR-CIK (Channel 1, green) and CD19 (Channel 2, red) were hybridized at 40 °C for 2 h using the HybEZ™ Hybridization System II. Signal amplification and detection utilized the RNAscope® Kit 2.5 HD Duplex Detection Kit (Cat. 322500), followed by red and green chromogenic revelation and Gill’s hematoxylin nuclear counterstaining. Slides were mounted using Vectamount® medium.



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    Singleplex in situ hybridization <t>(RNAscope)</t> showing detection of SGPV B22R1 and D13L transcripts in gills from Atlantic salmon at 1 and 3 days after exposure to SGPV (dpe). Whole gill serial sections from 1 dpe (n=3) were scanned for staining of B22R1 and D13L RNA, as marked (A, B) , revealing significant differences in detection of SGPV-positive cells (C) . Images of the SGPV-exposed sham injected (E.S) group from 1 dpe stained with B22R1 probe (D) and D13L probe (E) . Serial section from the E.S group from 3 dpe stained with B22R1 probe (F) and D13L probe (G) .
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    Image Search Results


    Singleplex in situ hybridization (RNAscope) showing detection of SGPV B22R1 and D13L transcripts in gills from Atlantic salmon at 1 and 3 days after exposure to SGPV (dpe). Whole gill serial sections from 1 dpe (n=3) were scanned for staining of B22R1 and D13L RNA, as marked (A, B) , revealing significant differences in detection of SGPV-positive cells (C) . Images of the SGPV-exposed sham injected (E.S) group from 1 dpe stained with B22R1 probe (D) and D13L probe (E) . Serial section from the E.S group from 3 dpe stained with B22R1 probe (F) and D13L probe (G) .

    Journal: Frontiers in Immunology

    Article Title: Mucosal and Systemic Immune Responses to Salmon Gill Poxvirus Infection in Atlantic Salmon Are Modulated Upon Hydrocortisone Injection

    doi: 10.3389/fimmu.2021.689302

    Figure Lengend Snippet: Singleplex in situ hybridization (RNAscope) showing detection of SGPV B22R1 and D13L transcripts in gills from Atlantic salmon at 1 and 3 days after exposure to SGPV (dpe). Whole gill serial sections from 1 dpe (n=3) were scanned for staining of B22R1 and D13L RNA, as marked (A, B) , revealing significant differences in detection of SGPV-positive cells (C) . Images of the SGPV-exposed sham injected (E.S) group from 1 dpe stained with B22R1 probe (D) and D13L probe (E) . Serial section from the E.S group from 3 dpe stained with B22R1 probe (F) and D13L probe (G) .

    Article Snippet: RNAscope ® 2.5 HD Duplex Detection Chromogenic kit (Advanced Cell Diagnostics) was used for simultaneous detection of SGPV-D13L and salmon GzmA in Atlantic salmon gills from all groups at 7 dpe (n (E.S) = 3, n (E.H) = 3, n (C.S) = 1, n (C.H) = 1) along with one section from the E.S group and the E.H group at 14 dpe.

    Techniques: In Situ Hybridization, RNAscope, Staining, Injection

    Duplex in situ hybridization (RNAscope) demonstrating the distribution of SGPV (D13L, blue staining) and salmon GzmA (red staining) in gills from Atlantic salmon. (A) E.S group at 7 dpe. (B) E.H group at 7 dpe. (C) C.S group at 7 dpe. (D) C.H group at 7 dpe. (E) E.S group from 14 dpe. (F) E.H group at 14 dpe. Arrow show interaction between GzmA expressing cell and SGPV-infected cell.

    Journal: Frontiers in Immunology

    Article Title: Mucosal and Systemic Immune Responses to Salmon Gill Poxvirus Infection in Atlantic Salmon Are Modulated Upon Hydrocortisone Injection

    doi: 10.3389/fimmu.2021.689302

    Figure Lengend Snippet: Duplex in situ hybridization (RNAscope) demonstrating the distribution of SGPV (D13L, blue staining) and salmon GzmA (red staining) in gills from Atlantic salmon. (A) E.S group at 7 dpe. (B) E.H group at 7 dpe. (C) C.S group at 7 dpe. (D) C.H group at 7 dpe. (E) E.S group from 14 dpe. (F) E.H group at 14 dpe. Arrow show interaction between GzmA expressing cell and SGPV-infected cell.

    Article Snippet: RNAscope ® 2.5 HD Duplex Detection Chromogenic kit (Advanced Cell Diagnostics) was used for simultaneous detection of SGPV-D13L and salmon GzmA in Atlantic salmon gills from all groups at 7 dpe (n (E.S) = 3, n (E.H) = 3, n (C.S) = 1, n (C.H) = 1) along with one section from the E.S group and the E.H group at 14 dpe.

    Techniques: In Situ Hybridization, RNAscope, Staining, Expressing, Infection

    In situ hybridization (RNAscope) targeting GzmA (stained in red) in spleen from Atlantic salmon at 14 dpe. (A) SGPV-exposed hydrocortisone-injected (E.H) group. (B) SGPV-exposed sham-injected (E.S) group.

    Journal: Frontiers in Immunology

    Article Title: Mucosal and Systemic Immune Responses to Salmon Gill Poxvirus Infection in Atlantic Salmon Are Modulated Upon Hydrocortisone Injection

    doi: 10.3389/fimmu.2021.689302

    Figure Lengend Snippet: In situ hybridization (RNAscope) targeting GzmA (stained in red) in spleen from Atlantic salmon at 14 dpe. (A) SGPV-exposed hydrocortisone-injected (E.H) group. (B) SGPV-exposed sham-injected (E.S) group.

    Article Snippet: RNAscope ® 2.5 HD Duplex Detection Chromogenic kit (Advanced Cell Diagnostics) was used for simultaneous detection of SGPV-D13L and salmon GzmA in Atlantic salmon gills from all groups at 7 dpe (n (E.S) = 3, n (E.H) = 3, n (C.S) = 1, n (C.H) = 1) along with one section from the E.S group and the E.H group at 14 dpe.

    Techniques: In Situ Hybridization, RNAscope, Staining, Injection