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rnascope2 5 hd duplex detection kit chromogenic  (Advanced Cell Diagnostics Inc)


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    Advanced Cell Diagnostics Inc rnascope2 5 hd duplex detection kit chromogenic
    Rnascope2 5 Hd Duplex Detection Kit Chromogenic, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope+2%2E5+hd+duplex+detection+kit+(chromogenic)/RNAscope+2%2E5+HD+Duplex+Reagent+Kit/bio_rxiv__64898__2026__07__02__735997-95-5-11
    Average 99 stars, based on 260 article reviews
    rnascope2 5 hd duplex detection kit chromogenic - by Bioz Stars, 2026-10
    99/100 stars

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    RNAscope:

    Article Title: Single Cell Transcriptomics Identifies Selective Lineage-Specific Regulation Of Genes in Aortic Smooth Muscle Cells in Mice
    Article Snippet: .. Tissue sections were pretreated according to the instructions from Advanced Cell Diagnostics (322380) and RNAscope protocol was performed using 2.5 HD duplex chromogenic detection kit (322500, ACD) and HybEZ II oven (321721, ACD). .. The mRNA probes (from ACD) used for RISH were Des (407921), Tnnt2 (418681), Galnt6 (48051), Hand2os1 (1151801-C2), Ccn5 (871701-C2).

    Article Title: Multimodal assessment of high-risk human papillomavirus in sinonasal squamous cell carcinoma
    Article Snippet: Viral hrHPV E6/E7 expression was detected via manual RNA ISH using the chromogenic RNAScope TM 2.5 HD Duplex Detection Kit with the HPV-HR18 probe array (Advanced Cell Diagnostics, Newark, CA ), which contains probes for E6/E7 mRNA from 18 hrHPV types (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) [ 34 , 43 , 46 ]. .. Viral hrHPV E6/E7 expression was detected via manual RNA ISH using the chromogenic RNAScope 2.5 HD Duplex Detection Kit with the HPV-HR18 probe array (Advanced Cell Diagnostics, Newark, CA ), which contains probes for E6/E7 mRNA from 18 hrHPV types (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) [ 34 , 43 , 46 ]. .. Unstained FFPE slides were prepared according to the manufacturer’s suggested protocol using a HybEZ Hybridization System (Advanced Cell Diagnostics).

    Article Title: Orexinergic responses to environmental stressors in the zebrafish gut
    Article Snippet: The target retrieval (RNAScopeTM Probe-Dr-hcrt, ACD Bio Cat. No. 887231) was done using the manual method described for 5 min. For the protease step, RNAScope ® Protease Plus was applied for 15 min. Brightfield images were acquired on a SLIDEVIEWTM VS200 research slide scanner (Evident Scientific, Waltham, MA, USA) using a color camera under 20X (UPlanXApo 20x/0.8 OFN26.5) and 40X objective (UPanXApo 40x/0.95 OFN26.5) magnification. .. Subsequent steps were performed according to ACD Bio (Newark, CA, USA) using RNAscope 2.5 Reagent Kit and kit manufacturer’s protocol Formalin-Fixed Parrafin-Embedded (FFPE) Sample Preparation and Pretreatment (ACD Bio document number 322452) and RNAscope 2.5 HD Duplex Detection Kit (Chromogenic) User Manual PART 2 (ACD Bio document number 322500-USM). .. The target retrieval (RNAScope Probe-Dr-hcrt, ACD Bio Cat. No. 887231) was done using the manual method described for 5 min. For the protease step, RNAScope Protease Plus was applied for 15 min. Brightfield images were acquired on a SLIDEVIEW VS200 research slide scanner (Evident Scientific, Waltham, MA, USA) using a color camera under 20X (UPlanXApo 20x/0.8 OFN26.5) and 40X objective (UPanXApo 40x/0.95 OFN26.5) magnification.

    Article Title: Postnatal Dynamic Ciliary ARL13B and ADCY3 Localization in the Mouse Brain
    Article Snippet: Tissue sections (15 μm) were collected using our fixation and preparation protocol above and then prepped and pretreated with 4% PFA for 16 h at 4 °C, followed by part 2 of protocol TN 320534 (Advanced Cell Diagnostics (ACD), Newark, CA, USA) as described [ ]. .. Following tissue preparation, the detection of transcripts was performed using an RNAscope 2.5 HD Duplex Detection Kit (Chromogenic) User Manual Part 2 (ACDBio Document 322500-USM, Newark, CA, USA). .. The slides were assayed using a probe specific to Arl13b (catalog no. 1044271-C2) transcripts (ACD), counterstained with hematoxylin, dehydrated, and mounted using VectaMount (Vectorlabs, Burlingame, CA, USA).

    Article Title: Primary cilia and BBS4 are required for postnatal pituitary development
    Article Snippet: Tissue cryosections (15 μm) were collected using our fixation and preparation protocol above and then prepped and pretreated with 4% PFA for 16 hours at 4°C, followed by part 2 of protocol TN 320534 (Advanced Cell Diagnostics (ACD), Newark, CA, USA) as described. .. Following tissue preparation, the detection of transcripts was performed using an RNAscope 2.5 HD Duplex Detection Kit (Chromogenic) User Manual Part 2 (ACDBio Document 322500-USM, Newark, CA, USA). .. The slides were assayed using a probe specific to Gli1 , Prl , Prop1 , Islet1 , Pax6 and Ptch1 transcripts (ACD), and counterstained with hematoxylin, dehydrated, and mounted using VectaMount (Vectorlabs, Burlingame, CA, USA), or counterstained with Hoechst and mounted with Prolong diamond for confocal fluorescence imaging.

    Article Title: Transcriptional profiling of retinal astrocytes identifies a specific marker and points to functional specialization
    Article Snippet: All the probes were purchased from Advanced Cell Diagnostics. .. In-situ hybridizations were carried out according to the manufacturer’s instructions using the RNAscope duplex detection chromogenic kit (Advanced Cell Diagnostics, #322500). .. All the probes were purchased from Advanced Cell Diagnostics.

    Article Title: Accessible spatial host–microbe profiling in tumour tissues
    Article Snippet: .. RNAScope assay was performed with RNAScope2.5 HD Duplex Detection Kit (Chromogenic) (Advanced Cell Diagnostics, Inc., Cat. No. 322430 and 322500) with RNAscope Probe-EB-16S-rRNA, C1 (Advanced Cell Diagnostics, Inc., Cat. No. 464461) and RNAscope Probe - B-Fusobacterium-23S-3zz-C2 (Advanced Cell Diagnostics, Inc., Cat. No. 486411-C2). .. Tissue slides were pretreated according to the user manual “Formalin-Fixed Paraffin-Embedded (FFPE) Sample Preparation and pretreatment” (ACDBio Document No. 322452), with slight modifications to the set up for target retrieval by using a heat-resistant plastic rack and water bath in place of a steamer.

    Expressing:

    Article Title: Multimodal assessment of high-risk human papillomavirus in sinonasal squamous cell carcinoma
    Article Snippet: Viral hrHPV E6/E7 expression was detected via manual RNA ISH using the chromogenic RNAScope TM 2.5 HD Duplex Detection Kit with the HPV-HR18 probe array (Advanced Cell Diagnostics, Newark, CA ), which contains probes for E6/E7 mRNA from 18 hrHPV types (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) [ 34 , 43 , 46 ]. .. Viral hrHPV E6/E7 expression was detected via manual RNA ISH using the chromogenic RNAScope 2.5 HD Duplex Detection Kit with the HPV-HR18 probe array (Advanced Cell Diagnostics, Newark, CA ), which contains probes for E6/E7 mRNA from 18 hrHPV types (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) [ 34 , 43 , 46 ]. .. Unstained FFPE slides were prepared according to the manufacturer’s suggested protocol using a HybEZ Hybridization System (Advanced Cell Diagnostics).

    In Situ Hybridization:

    Article Title: Multimodal assessment of high-risk human papillomavirus in sinonasal squamous cell carcinoma
    Article Snippet: Viral hrHPV E6/E7 expression was detected via manual RNA ISH using the chromogenic RNAScope TM 2.5 HD Duplex Detection Kit with the HPV-HR18 probe array (Advanced Cell Diagnostics, Newark, CA ), which contains probes for E6/E7 mRNA from 18 hrHPV types (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) [ 34 , 43 , 46 ]. .. Viral hrHPV E6/E7 expression was detected via manual RNA ISH using the chromogenic RNAScope 2.5 HD Duplex Detection Kit with the HPV-HR18 probe array (Advanced Cell Diagnostics, Newark, CA ), which contains probes for E6/E7 mRNA from 18 hrHPV types (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) [ 34 , 43 , 46 ]. .. Unstained FFPE slides were prepared according to the manufacturer’s suggested protocol using a HybEZ Hybridization System (Advanced Cell Diagnostics).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Orexinergic responses to environmental stressors in the zebrafish gut
    Article Snippet: The target retrieval (RNAScopeTM Probe-Dr-hcrt, ACD Bio Cat. No. 887231) was done using the manual method described for 5 min. For the protease step, RNAScope ® Protease Plus was applied for 15 min. Brightfield images were acquired on a SLIDEVIEWTM VS200 research slide scanner (Evident Scientific, Waltham, MA, USA) using a color camera under 20X (UPlanXApo 20x/0.8 OFN26.5) and 40X objective (UPanXApo 40x/0.95 OFN26.5) magnification. .. Subsequent steps were performed according to ACD Bio (Newark, CA, USA) using RNAscope 2.5 Reagent Kit and kit manufacturer’s protocol Formalin-Fixed Parrafin-Embedded (FFPE) Sample Preparation and Pretreatment (ACD Bio document number 322452) and RNAscope 2.5 HD Duplex Detection Kit (Chromogenic) User Manual PART 2 (ACD Bio document number 322500-USM). .. The target retrieval (RNAScope Probe-Dr-hcrt, ACD Bio Cat. No. 887231) was done using the manual method described for 5 min. For the protease step, RNAScope Protease Plus was applied for 15 min. Brightfield images were acquired on a SLIDEVIEW VS200 research slide scanner (Evident Scientific, Waltham, MA, USA) using a color camera under 20X (UPlanXApo 20x/0.8 OFN26.5) and 40X objective (UPanXApo 40x/0.95 OFN26.5) magnification.

    Sample Prep:

    Article Title: Orexinergic responses to environmental stressors in the zebrafish gut
    Article Snippet: The target retrieval (RNAScopeTM Probe-Dr-hcrt, ACD Bio Cat. No. 887231) was done using the manual method described for 5 min. For the protease step, RNAScope ® Protease Plus was applied for 15 min. Brightfield images were acquired on a SLIDEVIEWTM VS200 research slide scanner (Evident Scientific, Waltham, MA, USA) using a color camera under 20X (UPlanXApo 20x/0.8 OFN26.5) and 40X objective (UPanXApo 40x/0.95 OFN26.5) magnification. .. Subsequent steps were performed according to ACD Bio (Newark, CA, USA) using RNAscope 2.5 Reagent Kit and kit manufacturer’s protocol Formalin-Fixed Parrafin-Embedded (FFPE) Sample Preparation and Pretreatment (ACD Bio document number 322452) and RNAscope 2.5 HD Duplex Detection Kit (Chromogenic) User Manual PART 2 (ACD Bio document number 322500-USM). .. The target retrieval (RNAScope Probe-Dr-hcrt, ACD Bio Cat. No. 887231) was done using the manual method described for 5 min. For the protease step, RNAScope Protease Plus was applied for 15 min. Brightfield images were acquired on a SLIDEVIEW VS200 research slide scanner (Evident Scientific, Waltham, MA, USA) using a color camera under 20X (UPlanXApo 20x/0.8 OFN26.5) and 40X objective (UPanXApo 40x/0.95 OFN26.5) magnification.



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